As in standard western blotting, proteins are size separated via SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a blotting membrane ( Fig. The DigiWest approach combines standardized protein separation and western blotting with a multiplexed, bead-based immunoassay platform, such as the Luminex FlexMAP 3D system. An analysis of the expression of almost 200 proteins in tumour cells collected by laser capture microdissection from primary human mammary carcinoma demonstrates the capabilities of this approach for characterizing limited sample material.ĭescription of the DigiWest bead-based western blot We show that the sensitivity and reproducibility of this approach are as good as high-end western blotting systems and that the method is capable of providing high-resolution data on protein phosphorylation and expression. The digital nature of the resulting data prompted us to name this approach ‘DigiWest’. Accurate size information is obtained using western blot, and the advantages of a bead-based microarray platform-high throughput and low material consumption-are utilized to increase the output of this classical method. Here, we describe an approach that enables a highly parallel analysis of protein expression and modification status by adapting the classical western blot 13 to a bead-based microarray platform. High-throughput technologies often come with compromises in data quality, and highly reliable approaches such as western blotting are often required to subsequently confirm the results. This type of information helps provide a better understanding of cellular processes. Information on the presence of a wide variety of proteins can be obtained and, by detecting post-translational modifications, the activation states of regulatory cascades can be interrogated. Lower-throughput immunohistochemistry 10, high-content screening technologies 11 and reverse-phase protein microarrays (RPPAs) 12 allow for ‘systems biology approaches’ directly at the protein level. The proteome-wide generation of antibodies, driven by the Human Protein Atlas project 8 and other groups 9, opens up new possibilities and calls for immunoassay systems that are capable of performing multiple assays in parallel. By allowing the unbiased analysis of samples, mass spectrometry has had a massive impact on signalling research 6, as have immunoassay-based approaches 7. Although these breakthrough observations usually come from hypothesis-driven approaches, the development of new technologies 4 and advances in automation now result in new possibilities for understanding cellular signalling 5. Pathway dysregulation and aberrant cellular signalling have been linked to diseases such as cancer 2 and inflammatory disorders 3. Significant progress has been made in understanding cellular activity through the molecular analysis of signalling pathways 1. This high-throughput western blot approach allowed us to identify and characterize alterations in cellular signal transduction that occur during the development of resistance to the kinase inhibitor Lapatinib, revealing major changes in the activation state of Ephrin-mediated signalling and a central role for p53-controlled processes. The combination of molecular weight resolution, sensitivity and signal linearity on an automated platform enables the rapid quantification of hundreds of specific proteins and protein modifications in complex samples. By combining gel-based protein separation with immobilization on microspheres, hundreds of replicas of the initial blot are created, thus enabling the comprehensive analysis of limited material, such as cells collected by laser capture microdissection, and extending traditional western blotting to reach proteomic scales. The DigiWest approach we describe here transfers the western blot to a bead-based microarray platform. Dissecting cellular signalling requires the analysis of large number of proteins.
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#3 Stick to One Servingįor those of us who love having seconds, eating one serving will help us keep our calorie intake in check. To make this more measurable, write down how often you will make an effort to take at least a half hour to finish your meal. Take the extra time to pay attention to what we are eating and how much. It takes about 20 minutes for your brain to send out signals that you are full. To make this more measurable, write down how often you will make this choice e.g. Set a goal to drink water instead of sugar-sweetened drinks. For those of us with diabetes, these healthy eating habits will also help us to control our blood sugar. Here are 5 good eating habits which will definitely help us to achieve our food goals. To stay on track, we can break down these health goals into smaller and more realistic steps - steps that are easier to measure and track weekly or monthly so that we can see our progress and feel motivated.įor instance, we could set a smaller goal to develop a healthy eating habit such as drinking water instead of sweetened drinks during lunch. Exercising regularly could take years while eating healthier and making better food choices are hard to measure.īefore we achieve these goals, we may feel discouraged and give up. When it comes to health, we often have fitness and food goals like losing weight, exercising regularly, eating healthier or making better food choices. Often we write them down to make these goals more concrete and also as reminders to ourselves to keep moving towards our goals. They are expressions of our inner desires or what we want from our lives. We have goals for our relationships, careers, finance and also health. If you don’t like the results, tap Clear, then try again. On your device, use your finger or Apple Pencil (on iPad) to sign your name, then tap Done. If you don’t like the results, click Clear, then try again.Ĭreate a signature using your iPhone or iPad: Click Select Device to choose a device (if more than one is available). When your signature appears in the window, click Done. Hold your signature (on white paper) facing the camera so that your signature is level with the blue line in the window. If your Mac has a Force Touch trackpad, you can press your finger more firmly on the trackpad to sign with a heavier, darker line.Ĭreate a signature using your computer’s built-in camera: Click Camera. If you don’t like the results, click Clear, then try again. Ĭlick Create Signature if shown, then follow the onscreen instructions.Ĭreate a signature using your trackpad: Click Trackpad, click the text as prompted, sign your name on the trackpad using your finger, press any key, then click Done. In the Preview app on your Mac, click the Show Markup Toolbar button (if the Markup toolbar isn’t showing), then click the Sign button. To sign PDFs, you can capture your signature using your trackpad, the built-in camera on your Mac, or your iPhone or iPad. If Go to Page shows the wrong page of a PDF.
We ”PLUSED” up the new Mya Joy PLUS with additional lifestyle features that include a long-lasting rechargeable battery, USB charging cord and convenient wrist lanyard. The thoughtfully designed pump comfortably fits in the palm of a hand and has whisper quiet operation. Mya Joy PLUS combines the hospital strength performance moms demand with the incredibly small pump design they need. Meet the Ameda Mya Joy PLUS – the newest member of the Mya Joy family of pumping solutions. Join our community of Super Mom’s to ask questions and share your breast pumping experience.īundle Includes: BabyBuddha Smart & Rechargeable Breast Pump Unit (Black), Two (2) 24mm Flanges, Two (2) Sets of Duckbill Valves, One (1) Pair of Silicon Diaphragms, Two (2) Bottles, Two (2) Bottle Bases, Two (2) Bottle Storage Caps, Two (2) Bottle Hygiene Covers, Two (2) Pump Covers, Three (3) Detachable Tubes (for easy cleaning), USB Charging Cable (Charges to full in three hours), Detachable Lanyard, Carrying Tote Our unique suction pattern in stimulation mode mimics the suction pattern of a baby to increase breast milk production. Operating BabyBuddha is easy, simply recharge it and use the trackball to navigate through the 14 different modes, and land on the perfect one with a simple swivel of the thumb. Equipped with an impressively quiet mechanism, our breast pump keeps the whole process discreet. A breast pump allows you to have the best of both world.īabyBuddha’s double electric breast pump gives you freedom to move! The fully rechargeable pump is small enough to take anywhere. There are also some moms who prefer to feed their babies by a bottle when outside of the home, or who like to get the dads involved in the feeding process. In this case, breast pumps can be lifesavers for keeping your milk supply up and giving your baby the nutrients he or she needs to grow healthy and strong.īreast pumps are also important for building up an emergency milk supply if you ever need to be unexpectedly away from your baby. A pump allows you to feed your baby breastmilk through a bottle or small tube. If your baby was born prematurely, it’s possible that he or she is too small to breastfeed directly. Using a pump during those times when you do not have a hungry baby attached to you can help increase your production of milk and provide you with a back-up supply which you can then use to ease the breastfeeding process. Pumping while your baby is at the sitter or in daycare will help with engorgement and ensure you maintain your milk supply even when you are away from your baby for a part of the day.įor those moms with a low milk supply level, breastfeeding your baby can be a stressful ordeal. For working moms, a breast pump will allow you to continue to provide your baby with the health benefits of breast milk while you are away from the home. For those moms who decide to breast feed, you will find that a breast pump is an invaluable purchase. One decision that all pregnant moms will have to make is whether to breast feed or to formula feed their infant. Breastfeeding is the most natural way to provide the benefits of a mother’s milk to the growing and developing infant. I haven't said anything about this, at least I don't think I have. If I misunderstood your question, let me know, but this is a good faith effort to explain. But most apps these days allow you access and even export your data if your subscription is turned off.Īnyway, hopefully that helps. I do understand the argument about software that locks you out of your data if you stop using it-that's bullshit and shouldn't be allowed. I also can just stop using it and turn off the subscription if I find I no longer do things that require it. Now, I have the Adobe subscription, I get updates all the time and I don't have to go the rigamarole of going to a crowded mall, etc etc. I remember buying Adobe PS in the Apple store and walking out with it in my hand. I guess what confuses me most is that the cost to the consumer, in the long run, is about what it was in the era of software that you bought on a CD. Different per seat costs, different service tiers, different SLAs-very few businesses in the technology space, especially the small ones like we're talking about-are going to lock themselves out of being able to scale quickly when opportunity strikes. One of school of thought is, "Just figure out how much each of these things cost each year and work that into your expenses." Except, with SaaS products, it's not that simple. The folks who owned the data charged us something like $2k/month for access to it. I once did a project where we collected and normalized the test score information from a bunch of public schools. Geographic, analytic or demographic info that gets pulled into these things is usually from an API that it costs money to access. One of mine is an app that pulls in information on public officials-that's through an API using a service we pay for. Want to add devs to expand capacity quickly because you're behind or you're being opportunistic? Most of those costs start going up.Īnd that's hosting/storage/version control, ya know? The product that ends up in the client's hand has regular, ongoing costs as well. Hosting, and most companies who have multiple products are going to have multiple hosting providers, via Pantheon/WPE/AWS. Want to host a punch of different code repositories? Github. Then there's the stuff directly related to products. If you've got Salesforce (and you probably shouldn't because you probably don't need it), you're paying, usually, upwards of $40/seat/month. Slack, of course, and any specialty stuff like CRMs. In terms of sort of middleware, there's Dropbox/Box/GSuite, etc., all of which are monthly, recurring expenses per seat. (There's a number of others, but I don't do much on the operations side anymore so I don't remember off the top of my head.) Quicken, time tracking (Harvest), PM tools (Asana and the like), Mailchimp, any sort of social tools. Going back to my earlier comment, there's the basic stuff to run a business. I'm still in Mojave, and there seem to be many problems either with this OS (esp. The former makes me scroll down, starting over again. But again I must ask: After moving files to a folder, where should the cursor be reset? At beginning of the list of files/folders (which is how all programs do it that I know of)? OR should the cursor return to where you were in your list of files? For me, the latter makes more sense. I'm trying Commander One, and it's not bad. You can call me old if you like, and I guess I am getting old compared to many of you, but I don't see these moves as progress-not for serious people who use computers. Or its nagging "notifications." Or its demand that I "login" to the program ("create an account on our web site) merely to use a program which has nothing to do with the internet. I don't care for the loathsome "subscription model" the company has adopted. Turns out, it had an expiration date after all. I guess it's "I told you so" time for those of you who tried to warn me about Pathfinder. Linux Colordiff Command – Color diff Output wdiff Command You can check out the manual entry for colordiff as shown. You can install Colordiff on your Linux systems, using default package manager tools called yum, dnf, and apt-get or apt as shown. There are some wrappers for the diff tool that enhance its functionality and these include: colordiff CommandĬolordiff is a Perl script that produces the same output as diff, but with color and syntax highlighting. You can check out the manual entry for diff to easily use it. To compare two files and display the differences. Diff is simple and easy to use, it comes pre-installed on most Linux distributions, which compares files line by line and outputs the difference between them. I like to start with the original Unix command-line tool that shows you the difference between two computer files. There are several file comparison tools that you can use on Linux, and in this review, we shall look at some of the best terminal-based and GUI diff tools you can take advantage of while writing code or other text files. 9 Best Linux File Diff or Comparison Tools This description was born out of a reference to the output of diff, the well-known Unix command-line file comparison utility. When you compare two computer files on Linux, the difference between their contents is called a diff. While writing program files or normal text files, programmers and writers sometimes want to know the difference between two files or two versions of the same file.
As a dedicated therapist, I am committed to determining a treatment plan that accelerates your healing. Deep tissue is one of the most common massage modalities and Elements Massage. Deep, DmonJohn Varley, English Justices of the Peace, 1461-1509 (History/prehistory. Muscles easily become tense and deep tissue massage can relieve some chronic patterns of pain and tension, specifically in the back, shoulder and neck areas. Me Im A ToddlerSamantha Vickery, Collapse Weave: Creating Three. Smith has been practicing the art and science of massage therapy for nearly 20 years, specializing in connective tissue treatment and clearing trauma from the mind and the body. Swedish Massage, Deep Tissue Massage, Sports Massage, Hot Stone Massage, Focus Massage, Pregnancy Massage, Chair Massage, and Couples Massage. Deep tissue massage is generally a modality to consider for athletes as well as the eight-to-five desk job. The Alexander Method (r) of Vibrational Sound Therapy. If you are looking for a therapist that you can develop a therapeutic relationship with, I invite you to call to schedule an appointment. Specialties: We are specialists when it comes to massage Whether it's a relaxation massage or a therapeutic massage - Just Relax Massage & Spa is the place to go. Satisfaction with my treatments is very high as evidenced by client retention rates. Let your body tell me what it needs on any given day based upon assessments through palpation, intuition and subjective information that you provide. The ability to integrate Deep Tissue Massage, Myofascial Release / Body Memory Unwinding, Craniosacral Therapy and Medical Massage is the hallmark of each treatment plan. My work is very eclectic and far removed from a typical spa massage. The services menu incorporates several different healing modalities to accommodate your body’s unique needs for healing. With over 20 years of experience, I offer a full-service approach to wellness. My office is conveniently located in the Oyster Point area of Newport News and serves the Greater Peninsula area of Hampton Roads. I am a board certified (BCTMB) Massage Therapist licensed to conduct massage therapy in the State of Virginia. It can hold and run Apple scripts from a palette. yKey can open menu and choose menu items. An example of a command is: Open Photoshop and start a new document. All you have to do is put together three necessary parts of a shortcut: One or more commands that give the shortcut its functionality, a context in which it runs, and a launcher that defines how the shortcut is activated.Ī command is the action, or series of actions, that you want yKey to perform. In essence, an yKey shortcut is a little program in its own right, but you don’t need to know the first thing about programming to create an yKey shortcut. YKey is an automation utility, a program that creates shortcuts to accomplish repetitive tasks. Learn more about shortcuts in our overview section. Its all about efficiency, we call this “doing less and accomplishing more.” yKey can turn some repetitive action that might consist of thousands of mouse clicks (in various applications, in menus and different dialog boxes) and pages of typing into one yKey shortcut that can be launched anytime to perform the exact same action with a simple key press. YKey adds an essential but missing element in Mac OS X, automation! yKey will save you and your company precious time and energy. Seriously, why sacrifice your life to monotonous repetitive actions when your computer can do them for you. Instead of performing this same task each day for 15 mins with typing and clicking and opening and closing applications do it with yKey via one hotkey or menu item and let it work in the background while you do some other work or have a cup of tea. Say you open Safari each day and go to the same 8 websites and then copy some text which you email to someone. A simple example of how yKey can save you time! Rule your computer, don’t be a slave anymore, use yKey automation for mac. Free your self from the ball and chain of repetitive drudgery. We know computers are very capable of dealing with repitition, but there has been no easy way, up until now to collect them and pass them on to the computer to perform. We love our computers, but we are all familiar with the fatigue of repetitive tasks. Works with Ke圜ue which gives quick access to all your hotkeys. For those looking for an affordable solution to creating shortcuts in Mac OS X, I highly recommend taking a look at yKey.” - James Richvalsky Overall, I found the yKey to be reliable and very easy to use. “Since I started using yKey, this gem has saved me up to an hour a week using shortcuts. The iKey name was changed yKey a couple years after his review. More Ableton Live and yKey techniques here.īen Waldie’s tutorial is a great intro to yKey as automation/macro software.ĭavid Pogue discusses automation/macro software and in his weekly video he says yKey is his favorite. This is essential if you work and spend a lot of time with audio. March 2013 Tutorial “Using Ableton Live with yKey”įran Cotton’s in depth tutorial shows why yKey is essential for using Ableton Live 9. YKey was called iKey for it’s first decade then the name was changed to yKey. Engst is the author of the “Take Control of yKey” Manual. His manual helps you install yKey and provides a step by step usage tutorial for this essential Mac automation software.Īdam started the TidBits newsletter and has written numerous technical books, including the best-selling Internet Starter Kit series, and many magazine articles. 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